Composite

Part:BBa_K4452013

Designed by: iGEM22_Hopkins   Group: iGEM22_Hopkins   (2022-09-30)


Ferritin with prSGR9 transit peptide

Pyrococcus furiosus ferritin (PFt), an efficient iron storage protein, linked to GFP with expression under a columella cell-specific promoter, PLT-2, and with an N-terminus signal peptide to imports the ferritin into statoliths.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 95
    Illegal BglII site found at 527
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2370
    Illegal BsaI.rc site found at 2649


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